Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2) (N-Term) Peptide
ACCN2
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN972801
Quick Overview for Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2) (N-Term) Peptide (ABIN972801)
Target
ASIC1 (ACCN2)
(Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- N-Term
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-ACCN2 antibody (Catalog #: ARP35298_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))
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Background
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ACCN2 is the cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ACCN2 is also permeable for Ca2+, Li+ and K+. ACCN2 generates a biphasic current with a fast inactivating and a slow sustained phase. ACCN2 mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. ACCN2 functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. It modulates activity in the circuits underlying innate fear.This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit, however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products.
Alias Symbols: ASIC, ASIC1A, BNaC2, hBNaC2, ACCN2
Protein Interaction Partner: PICK1,ACCN1,ACCN2,PICK1,PRKACA,STOM,PICK1,PRKACA,STOML1
Protein Size: 574 -
Molecular Weight
- 65 kDa
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Gene ID
- 41
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NCBI Accession
- NM_020039, NP_064423
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UniProt
- P78348
Target
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